15During a long-term ecological study, students examined organisms collected from three different environments: a freshwater pond, damp soil near decaying logs and the digestive tract of animals. Scientists recorded only structural, cellular and nutritional features. P: microscopic; no true nucleus; rigid cell covering; survives high salinity and temperature. Q: multicellular; filamentous body; cell wall present; no chlorophyll; grows on dead organic matter. R: unicellular; true nucleus; contractile vacuole present; moves using flagella; photosynthesis in light but heterotrophic in the absence of light. S: multicellular; well-differentiated tissues; backbone present; aquatic respiration during early life stage. T: acellular; contains genetic material; remains inactive outside a host cell. (i) Identify one organism that clearly belongs to Kingdom Fungi. State one observation that supports your answer. (ii) Which organism would be placed in Kingdom Monera? Mention one characteristic that justifies this placement. (iii) Organisms R and Q are both eukaryotic, yet they are placed in different kingdoms. Analyse the criteria that separate them. (iv) Explain why organism S cannot be classified using the mode of nutrition alone. (v) Organism T does not fit into any of the five kingdoms. Which fundamental characteristic used in classification does it lack and what does this reveal about the limitations of classification systems? (vi) If classification were based only on habitat, which organisms might be incorrectly grouped together? Explain the scientific consequences of such a classification. (vii) Imagine scientists discover a new organism that is multicellular, eukaryotic, lacks chlorophyll and absorbs nutrients from a host externally. Should it be placed under fungi or animalia? Justify your reasoning using classification criteria.
Solution
(i) Q belongs to Fungi: it is multicellular and filamentous with a cell wall, has no chlorophyll and grows on dead organic matter (saprophytic), like a mould.
(ii) P belongs to Monera: it has no true nucleus (prokaryotic). Its rigid cell covering and survival in high salinity and temperature suggest an archaean or bacterium.
(iii) Both are eukaryotes, but they differ in other criteria:
| Criterion | R | Q |
| Level of organisation | Unicellular | Multicellular (filamentous) |
| Cell wall | Not mentioned (moves with flagella, has a contractile vacuole) | Present |
| Nutrition | Autotrophic in light, heterotrophic in dark | Heterotrophic only (saprophytic, no chlorophyll) |
| Movement | Moves using flagella | Does not move |
| Kingdom | Protista (like Euglena) | Fungi |
So the level of organisation (unicellular vs multicellular) and the mode of nutrition (and cell structure) separate them.
(iv) S is heterotrophic, but heterotrophic nutrition is shared by animals, fungi, many protists and most bacteria. Nutrition alone would put S together with moulds and bacteria. S must be classified using its other features: multicellular, with well-differentiated tissues, no cell wall and a backbone (Animalia, phylum Chordata, a vertebrate). Its aquatic respiration in early life suggests an amphibian such as a frog.
(v) T lacks cellular organisation; it is acellular, so none of the criteria (cell type, cell wall, level of organisation, mode of nutrition) can be applied to it. This shows that classification systems are built on chosen criteria and present knowledge: they cannot place everything (there is no place for acellular entities such as viruses), and they need to be revised as new discoveries are made.
(vi) Pond: R (a protist) and S (a frog, which lives in water in its early stage) would be grouped together, though one is a unicellular protist and the other a vertebrate. Damp soil near decaying logs: Q (a fungus) would be grouped with soil bacteria such as P. Digestive tract: bacteria and protists would be grouped with each other. Also, the same organism can live in several habitats (e.g. frogs on land and in water, bacteria everywhere), so it would be put in more than one group. Consequences: unrelated organisms would be grouped together and related ones separated; the groups would not reflect structure, cell type or evolutionary relationships; and we could not predict an organism's features from its group, so the classification would be scientifically useless.
(vii) Fungi. It is multicellular and eukaryotic, which fits both kingdoms, and lacks chlorophyll, so it is heterotrophic, also true of both. The deciding criterion is how it obtains food: it absorbs nutrients from outside its body (from the host), which is the absorptive (saprophytic or parasitic) nutrition of fungi. Animals ingest food and digest it inside the body. To confirm, the scientist should check for a cell wall made of chitin (fungi) as against no cell wall (animals).
(i) Q (no chlorophyll, grows on dead matter, cell wall) (ii) P (no true nucleus) (iii) R is unicellular, motile and photosynthetic (Protista); Q is multicellular, non-motile and saprophytic (Fungi) (iv) Heterotrophy is shared by animals, fungi, protists and bacteria; S needs features like tissues and backbone (v) Cellular organisation; classification systems cannot place acellular entities (vi) e.g. R and S (pond), P and Q (soil): unrelated organisms grouped together (vii) Fungi: absorptive nutrition (check for a chitin cell wall).